Stool Culture – Basic microbiology, parasitology, and immunology; nature, reproduction, growth, and transmission of common microorganisms and parasites in Bangladesh; prevention including universal precaution and immunization, control, sterilization, and disinfection; and specimen collections and examination. Students will have an understanding of common organisms and parasites caused human diseases and acquire knowledge about the prevention and control of those organisms.
Stool Culture
Media Used For Stool Culture
- Mac Conkey’s agar media.
- XLD (Xylose Lysine Deoxycholate).
- DCA (Deoxycholate Citrate agar).
- Tetrathionate broth (Selenite F broth).
Procedure of Stool Collection for Culture
A. Articles:
- A clean specimen container
- A spatula for putting the specimen into the container
- Dry bed pan (for helpless patients). Additional bedpan for rinsing and cleaning
- Laboratory requisition form.
- Clean gloves.
- Waste paper (for wrapping used spatula).
- A pitcher of water (for helpless patient).
- Tissue/towel.
B. Procedure:
1. Check the physicians order.
2. Identify the patient.
3. Explain to patient the procedure and make clear what is expected of him/her.
4. Give the labeled container and spatula to the patient with instructions:
5. To defecate into clean bedpan.
6. Not to contaminate specimen with urine.dgovo
7. Don gloves.
8. For helpless patient: assist patient on to the clean bedpan.
9. Leave him with instructions that has
10. When done, remove and keep aside the bedpan after placing the second one for cleansing.
11. Once the specimen is collected sent it to lab with the appropriate requisition forms.
12. Wash and replace the reusable articles.
13. Dispose of the used spatula wrapped in waste paper.
14. Wash and dry hands.
15. Record information in the patient’s chart.
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