Concept about Urine Culture – Basic microbiology, parasitology, and immunology; nature, reproduction, growth, and transmission of common microorganisms and parasites in Bangladesh; prevention including universal precaution and immunization, control, sterilization, and disinfection; and specimen collections and examination. Students will have an understanding of common organisms and parasites caused human diseases and acquire knowledge about the prevention and control of those organisms.
Concept about Urine Culture
Indication of Culture of Urine
To diagnose the following diseases-
- Urinary tract infection (UTI).
- Enteric fever.
- PUO (pyrexia of unknown origin).
- AGN (acute glomerulonephritis) as post-streptococcal sequalae.

Procedure of Urine Collection for Culture
A. Equipment:
1. Clean, wide mouthed container,
2. Laboratory requisition form.
3. Bed pan or urinal.
4. Soap and water.
5. Appropriate laboratory form.
6. Gloves.

B. Procedure:
1. Check the physician order and nursing care plan. old mo
2. Identify the patient.
3. Explain procedure to the patient with specific instructions about washing the genital area (skin around the urethra meatus) with soap and water and give the labeled container. Instruct patient not wet the label on the outside.
4. Ask the patient to direct the first and last part of the urine stream into a urinal or toilet and to collect the middle part of the stream into the special container.
5. Have the patient place the specimen container in proper / designated place.
6. With gloved hand place the specimen container in polythene bag.
7. Send specimen to the laboratory with completed, signed laboratory form.
8. Remove gloves and wash hands.
9. Record the procedure in the nurse’s notes and other appropriate forms.
