Today our topic of discussion is Procedure for Amniotic Fluid Analysis.
Procedure for Amniotic Fluid Analysis
Procedure
- A consent form should signed
- Food and fluids are not restricted
- Have a client to void before the procedure to prevent puncturing the bladder and aspirating urine
- Cleanse the suprapubic area with an antiseptic such as povidone-iodine (betadine).
- A local anesthetic is injected at the site for amniocentesis
- The placenta and fetus should be located by ultrasound or manually (fetus only).
- A 22 gauge spinal needle with sylet is inserted through the skin to the amniotic cavity
- 5-15 mL amniotic fluid is aspirated. Apply a small dressing to the needle insertion site
- The procedure takes approximately 30 minutes.
Factors Affecting Diagnostic Results
A traumatic amniotic tap may produce blood in the amniotic fluid.

Nursing Implications
- Recognize when amniocentesis for amniotic fluid analysis is indicated (e.g. with a familial history of sex- linked, genetic or chromosomal disorders; with a history of previous miscarriages; and in advanced maternal age (>35-years-old). It is not a screening test
- Be supportive of the women and her partner. Be a good listener. Allow them time to ask questions and to express any concerns. Refer questions you cannot answer to the appropriate health professionals
- Be sure that the client urinates before the test and that the consent form is signed.
Client Teaching
- Inform the client that normal results do not guarantee a normal infant, nor do they always predict sex correctly. The health care provider should tell the woman of potential risks
- Instruct the client to notify the healthcare provider immediately of any of the following: bleeding or leaking fluid from the vagina, abdominal pain or cramping, chills and fever or lack of fetal movement
- Encourage the women and her partner to seek genetic counseling especially if a chromosomal abnormality has been detected.
Rare Complications
- Premature labor
- Spontaneous abortion
- Infection
- Fetal or placental bleeding.
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