Stool Routine Test and Culture

Today is our topic of discussion is Stool Routine Test and Culture.

Stool Routine Test and Culture

 

STOOL-ROUTINE TEST AND CULTURE

Collection of stool specimen for specific or routine tests- (stool culture to detect abnormal characteristics). Steps in stood collecting procedure is discussed in

Purpose

  • To identify specific pathogens
  • To determine presence of blood, ova and parasites
  • To determine presence of fat
  • To do gross examination of stool characteristics such as color, consistency and odor.

Normal Characteristics of Feces

  • Color: Light to dark brown
  • Odor: Pungent smell
  • Frequency: 1-2 times per day
  • Quantity: 4-5 ounces per day
  • Composition: 30% water, shed epithelium from the intestine, a considerable quantity of bacteria and a small quantity of nitrogenous matter
  • Stool of infants: At birth, the stool of infants is dark green and it is called “meconium”

 

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Abnormal Characteristics of Feces

Color

  • Tarry black stools-bleeding in the upper gastro- intestinal tract
  • Black color stool-melena, administration of iron or
  • charcoal
  • Clay colored stool-obstruction to the flow of bile
  • White colored stool-presence of barium salts after barium tests.

Odor

  • Melena and dysentery-foul smell.
  • Frequency
  • Diarrhea- increased frequency
  • Constipation-decreased in frequency and low residue diet.

Consistency and Form

  • Watery stools-diarrhea
  • Rice water stools – cholera
  • Pea soup stools-typical of typhoid fever.

Appearance

  • Fresh blood in large amounts-bleeding piles
  • Blood and mucus stool-amoebic or bacillary dysentery
  • Worm or segments or worms in stool-parasitic cysts, ova or larvae.

 

Stool Routine Test and Culture

 

General Instructions

  • Fecal specimens are collected for chemical bacterio- logical or parasitological analysis .
  • Fecal specimens should be collected in the early stages of disease preferably before antibiotic treatment is given .
  • Stool specimens should be collected in a sterile container (making use of the scoop provided in the container) with a tight-fitting leak proof-lid (Fig. 23.6).
  • After collection, the lid should be immediately replaced tightly.
  • After proper labeling, the collected stool should be handed over to the laboratory without delay.

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